human icam1 cd54 allele specific quantikine elisa kit  (R&D Systems)

Journal: bioRxiv

Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7

doi: 10.1101/2025.10.28.685237

Figure Lengend Snippet: Impact of ADAMTS-7 deficiency on vascular smooth muscle cell supernatants secondary to incubation with CCL17. A . Representative cytokine profiler blots (left) and heatmap visualizing cytokine profiler results. Cytokines displaying a more than 50% difference between the conditions are highlighted in blue. CCL17 was similar between the groups (red). B . Validation of findings from A using enzyme-linked immunosorbent assays (n=6 each). Each symbol indicates one independent experiment. Data are mean and s.e.m. Unpaired t-test. Abbreviations: CCL20 , C-C motif chemokine 20; FC , fold-change; GM-CSF , Granulocyte-Macrophage Colony-Stimulating Factor; HGF, Hepatocyte Growth Factor; ICAM1 , intercellular adhesion molecule 1; IL-1β , Interleukin 1-β; MCP - 3 , Monocyte Chemotactic Protein 3; scr , scramble; TS7 , ADAMTS-7; VEGF , Vascular Endothelial Growth Factor.

Article Snippet: In cell culture supernatants, GM-CSF (DGM00), IL-1β (DLB50), CCL7 (DCC700), VEGF (DVE00), HGF (DHG00B), ICAM1 (DCD540), and CCL20 (DM3A00; all from R&D Systems) were used to quantify cytokine concentrations.

Techniques: Incubation, Biomarker Discovery

Journal: bioRxiv

Article Title: Cigarette smoking upregulates vascular expression of the novel atherosclerosis risk factor ADAMTS-7

doi: 10.1101/2025.10.28.685237

Figure Lengend Snippet: Reduced activation of endothelial cells by conditioned media of vascular smooth muscle cells lacking ADAMTS-7. A . Schematic overview of the experimental setup. B . Significantly downregulated adhesion molecules in the absence of ADAMTS-7 (n=7 each). C . Reduced adhesion of THP-1 cells to endothelial cells in the absence of ADAMTS-7 (n=8 each). Each symbol indicates one independent experiment. Data are mean and s.e.m. Unpaired t-test. Abbreviations: CDH5 , VE-cadherin; FC , fold-change; EC , endothelial cell(s); ICAM1 , intercellular adhesion molecule 1; ICAM2 , intercellular adhesion molecule 2; SELE , E-selectin; TS7 , ADAMTS-7; VCAM1 , vascular cell adhesion molecule 1; VSMC , vascular smooth muscle cell(s).

Article Snippet: In cell culture supernatants, GM-CSF (DGM00), IL-1β (DLB50), CCL7 (DCC700), VEGF (DVE00), HGF (DHG00B), ICAM1 (DCD540), and CCL20 (DM3A00; all from R&D Systems) were used to quantify cytokine concentrations.

Techniques: Activation Assay